Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods


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High Growth Potential of Long-Term Starved Deep Ocean Opportunistic Heterotrophic Bacteria

Introduction: Single cell biotechnology for uncultured microorganisms. Localisation of proteins within intact bacterial cells using fluorescent protein fusions. Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division. Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging BONCAT.


  • Gordon and Betty Moore Foundation Investigator (Grant #3780).
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Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity. Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence. There are no comments yet.

Multi-Omics Measurements of Single Samples - mSystems

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Visualizing in situ translational activity for identifying and sorting slow-growing archaeal-bacterial consortia. Proc Natl Acad Sci U. Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging BONCAT. Methyloprofundus sedimenti gen. Enrichment and genome sequence of the group I.

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Diversity, physiology and niche differentiation of ammonia-oxidizing archaea. The genome of the ammonia-oxidizing Candidatus Nitrososphaera gargensis: Insights into metabolic versatility and environmental adaptations. Thaumarchaeotes abundant in refinery nitrifying sludges express amoA but are not obligate autotrophic ammonia oxidizers.

Demonstrates that some amoA gene-carrying and expressing group I. Multicellular photo-magnetotactic bacteria. Distinct gene set in two different lineages of ammonia-oxidizing archaea supports the phylum Thaumarchaeota.

Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods
Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods
Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods
Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods
Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods
Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods

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